{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_8372621_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"093-309-146-907-481","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10838,"type":"PATENT","title":"Michigan State University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":5685,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8287,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: Michigan AND State AND Univ*
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4400
Search Applicants and Owners separately: Michigan AND State AND Univ*
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4400
1) providing\n\n(A) erythrose 4-phosphate (E4P) and pyruvate,\n(B) an isolated or recombinant 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) aldolase having the amino acid sequence of SEQ ID NO:2, and,\n(C) an aqueous medium,\n
2) contacting in said medium, said KDPGal aldolase with said E4P and said pyruvate under conditions suitable for said KDPGal aldolase to catalyze the formation of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) from the E4P and pyruvate."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["A method for converting pyruvate and erythrose 4-phosphate (E4P) to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP), comprising contacting an isolated or recombinant 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) aldolase having the amino acid sequence of SEQ ID NO:2 with pyruvate and E4P, wherein said contacting converts pyruvate and E4P to DAHP, wherein the recombinant KDPGal aldolase comprises at least one mutation selected from the group consisting of X10V, X28L, X28M, X42T, X85A, X154F, and X1961."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein said contacting step is performed in vivo in a recombinant cell."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, further comprising obtaining recombinant KDPGal aldolase from a recombinant cell, and contacting the KDPGal aldolase with pyruvate and E4P in solution to convert pyruvate and E4P to DAHP."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein the recombinant KDPGal aldolase has a specific activity for DAHP formation in the range of 0.3 U/mg to 1.3 U/mg."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A method for converting pyruvate and erythrose 4-phosphate (E4P) to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP), comprising contacting an isolated or recombinant 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) aldolase having the amino acid sequence of SEQ ID NO:2 with pyruvate and E4P, wherein said contacting converts pyruvate and E4P to DAHP, wherein the recombinant KDPGal aldolase comprises at least one mutation selected from the group consisting of X10V, X28L, X28M, X42T, X85A, X154F, and X1961, and wherein said recombinant KDPGal aldolase has higher specific activity for 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) formation than a native KDPGal aldolase without said at least one mutation."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein said at least one mutation is selected from the group consisting of S42T, V85A, and V154F."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein the recombinant KDPGal aldolase has no mutation that is X70L."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 9 further comprising recovering DAHP from said medium."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 9 further comprising:\n
providing a 3-dehydroquinate (DHQ) synthase, and\n
contacting said DAHP with said DHQ synthase under conditions suitable for said DHQ synthase to catalyze the formation of 3-dehydroquinate from the DAHP."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 18 further comprising recovering at least one of DAHP, DHQ, 3-dehydroshikimate (DHS), from said medium."],"number":19,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}