{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"EP_2455878_A2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"006-500-600-616-415","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11639,"type":"PATENT","title":"Seoul National University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":10325,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8305,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
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Add to collection. Total patents: 8610
Search Applicants and Owners separately:Seoul univ*
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Add to collection. Total patents: 8610
1) synthesizing cDNA from RNA of a subject;
2) performing real-time PCR to amplify the target gene using a pair of primers and/or probes with the cDNA serving as a template and then performing real-time PCR to amplify at least one of Endogenous Reference Genes selected from a group consisting of Accession No. Hs 500775(ZNF207), Accession No. Hs 446427(OAZ1), Accession No. Hs 530118(LUC7L2), Accession No. Hs 208597(CTBP1), Accession No. Hs 440382(TRIM27), Accession No. Hs 444279(GPBP1), Accession No. Hs 250009(ARL8B), Accession No. Hs 9589(UBQLN1), Accession No. Hs 253726(PAPOLA), Accession No. Hs 146806(CUL1), Accession No. Hs 533222(DIMT1L), Accession No. Hs 494985(FBXW2) and Accession No. Hs 242458(SPG21); and
3) normalizing an expression level of the target gene to that of the endogenous reference gene of step 2)."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the Endogenous Reference Genes are amplified by a pair of primers and/or probes."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the Endogenous Reference Genes show low expression levels, similar to most low-abundance intracellular transcripts."],"number":3,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}