{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"EP_1295951_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"075-465-071-022-655","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8906,"type":"PATENT","title":"Univ Pennsylvania Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":24238,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8221,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
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(a) providing cDNA obtained by reverse transcription of mRNA of the test cell;
(b) providing a collection of cultured cells of said library organized into individual clones, wherein each clone is of a cell having a mutation in an exon in its genome, the mutation being in a different exon in cells of different clones;
(c) providing an array of different single stranded polynucleotides, the polynucleotides being fragments of exons containing mutations in (b);
(d) exposing the cDNA to the array under conditions permitting hybridization of polynucleotides in the array to nucleic acids;
(e) detecting hybridization of cDNA to a polynucleotide on the array; and,
(f) selecting a clone in the collection from which a hybridizing polynucleotide detected at (c) is an exon fragment."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["A method for comparing gene expression between test cells, comprising:
(a) providing at least two cDNA samples, each sample obtained by reverse transcription of mRNA of a different test cell;
(b) providing a collection of cultured cells of a cell library organized into individual clones, wherein each clone is of a cell having a mutation in an exon of its genome, the mutation being in a different exon in cells of different clones;
(c) providing at least one array of different single stranded polynucleotides, the polynucleotides being fragments of exons containing mutations in (b);
(d) exposing the cDNA samples to the at least one array under conditions permitting hybridization of polynucleotides on the array to nucleic acids;
(e) detecting hybridization of polynucleotides in the at least one array resulting from exposure to cDNA;
(f) selecting clones in the collection from which hybridizing polynucleotides detected at (e) are exon fragments; and,
(g) comparing a clone or clones which comprise exon fragments that hybridize to one of the cDNA samples to a clone or clones which comprise exon fragments that hybridize to another of the cDNA samples."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 or claim 2, wherein mutations in the cell library are as a result of introducing an exon trap vector into the source cells of said library."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 1 to 3, wherein the source cells of said cell library are murine."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 1 to 4, wherein the cells of said cell library are ES cells."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 1 to 5, wherein the array is a nucleic acid microarray."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein the microarray comprises at least 500 different polynucleotides on a solid support surface."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein the microarray comprises at least about 1,000 different polynucleotides."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 1-8, wherein the cDNA is labelled to facilitate detection at (e)."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein the label is fluorescent or radioactive."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 1-10, wherein selecting a clone comprises physically segregating a sample of cells from a selected clone."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A system for testing expression of a gene in a test cell, comprising:
(a) a collection of cultured cells of a cell library organized into individual clones, wherein each clone is of a cell having a mutation in an exon of its genome, the mutation being in a different exon in cells of different clones; and,
(b) an array comprising at least 500 different single stranded polynucleotides on a solid support surface, the polynucleotides being fragments of the exons containing mutations in (a)."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 12, wherein the cells of said cell library are ES cells"],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 12 or claim 13, wherein the array comprises at least about 1,000 different polynucleotides."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 14, wherein the array comprises at least about 10,000 different polynucleotides."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The system of any one of claims 12 to 15, wherein the array is a nucleic acid microarray."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The system of any one of claims 12 to 16, wherein the system additionally comprises a recorded index associating a position in the array at which a polynucleotide is present, to a clone comprising that polynucleotide in an exon in which there is a mutation."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 17, wherein the recorded index is stored on a computer-readable medium."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The system of any one of claims 13 to 18, wherein the ES cells are murine."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["An exon trap vector comprising, in a 5' to 3' direction:
(a) an unpaired splice acceptor;
(b) a region encoding a reporter;
(c) one or more polyadenylation signals;
(d) a promoter functional in an ES cell;
(e) a segment encoding a second reporter under transcriptional control of promoter (d); and,
(f) an unpaired splice donor,wherein the construct additionally comprises a selectable region of 300 base pairs or less between (a) and (b) or between (e) and (f)."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The vector of claim 20, wherein the selectable region encodes a selectable marker."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The vector of claim 20, wherein the selectable region is supF."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The vector of claim 20, wherein the selectable region is a recombination site."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The vector of claim 23, wherein the recombination site is selected from the group consisting of: att, lox, and frt."],"number":24,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}